Recombinant Human AURKB, His-tagged

• Cat.No.: AURKB-27422TH
• Product Overview: Recombinant fragment, corresponding to amino acids 2-344 of Human Aurora B with a proprietary enzyme and His tag

Specification

• Species: Human
• Tag: His
• Protein Length: 2-344 a.a.
• Description: Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al.
• Conjugation: HIS
• Tissue specificity: High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
• Biological activity: 12 U/ug. One unit is defined as the amount of enzyme that willphosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 4 uM au
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: AQKENSYPW PYGRQTAPSG LSTLPQRVLR KEPVTPSALV LMSRSNVQPT AAPGQKVMEN SSGTPDILTR HFTIDDFEIG RPLGKGKFGN VYLAREKKSH FIVALKVLFK SQIEKEGVEH QLRREIEIQA HLHHPNILRL YNYFYDRRRI YLILEYAPRG ELYKELQKSC TFDEQRTATI MEELADALMY CHGKKVIHRD IKPENLLLGL KGELKIADFG WSVHAPSLRR KTMCGTLDYL PPEMIEGRMH NEKVDLWCIG VLCYELLVGN PPFESASHNE TYRRIVKVDL KFPASVPTGA QDLISKLLRH NPSERLPLAQ VSAHPWVRAN SRRVLPPSAL QSVA
• Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.Contains 1 protein kinase domain.

Gene Information

• Gene Name: AURKB aurora kinase B [ Homo sapiens ]
• Official Symbol: AURKB
• Synonyms: AURKB; aurora kinase B; serine/threonine kinase 12 , STK12; Aik2; AIM 1; ARK2; AurB; aurora 1; aurora B; IPL1; PPP1R48; protein phosphatase 1; regulatory subunit 48; STK5
• Gene ID: 9212
• mRNA Refseq: NM_004217
• Protein Refseq: NP_004208
• MIM: 604970
• Uniprot ID: Q96GD4
• Chromosome Location: 17p13.1
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Aurora C signaling, organism-specific biosystem
• Function: ATP binding; metal ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity