Recombinant Human AUH protein, GST-tagged

• Cat.No.: AUH-1035H
• Product Overview: Human AUH partial ORF ( NP_001689, 44 a.a. - 135 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes bifunctional mitochondrial protein that has both RNA-binding and hydratase activities. The encoded protein is a methylglutaconyl-CoA hydratase that catalyzes the hydration of 3-methylglutaconyl-CoA to 3-hydroxy-3-methyl-glutaryl-CoA, a critical step in the leucine degradation pathway. This protein also binds AU-rich elements (AREs) found in the 3' UTRs of rapidly decaying mRNAs including c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. This protein is localizes to the mitochondrial matrix and the inner mitochondrial membrane and may be involved in mitochondrial protein synthesis. Mutations in this gene are the cause of 3-methylglutaconic aciduria, type I. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 35.86 kDa
• AA Sequence: RAGPAIWAQGWVPAAGGPAPKRGYSSEMKTEDELRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPG
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: AUH AU RNA binding protein/enoyl-CoA hydratase [ Homo sapiens ]
• Official Symbol: AUH
• Synonyms: AUH; AU RNA binding protein/enoyl-CoA hydratase; AU RNA binding protein/enoyl Coenzyme A hydratase , AU RNA binding protein/enoyl Coenzyme A hydratase; methylglutaconyl-CoA hydratase, mitochondrial; 3-methylglutaconyl-CoA hydratase; AU-binding protein/Enoyl-CoA hydratase; AU-specific RNA-binding enoyl-CoA hydratase; AU RNA binding protein/enoyl-Coenzyme A hydratase; AU RNA-binding protein/enoyl-Coenzyme A hydratase
• Gene ID: 549
• mRNA Refseq: NM_001698
• Protein Refseq: NP_001689
• MIM: 600529
• UniProt ID: Q13825