Recombinant Human AUH, 68-339 aa, His-tagged

• Cat.No.: AUH-3384H
• Product Overview: Recombinant human AUH protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography.MW = 31.4 kDa (293aa).

Specification

• Cat. No.: AUH-3384H
• Description: AUH, also known as mitochondrial methylglutaconyl-CoA hydratase, is involved in the amino acid degradation pathway by catalyzing the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and water. It has been found to have very low enoyl-CoA hydratase activity.
• Source: E.coli.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSSEMKTEDE LRVRHLEEEN RGIVVLGINR AYGKNSLSKN LIKMLSKAVD ALKSDKKVRT IIIRSEVPGI FCAGADLKER AKMSSSEVGP FVSKIRAVIN DIANLPVPTI AAIDGLALGG GLELALACDI RVAASSAKMG LVETKLAIIP GGGGTQRLPR AIGMSLAKEL IFSARVLDGK EAKAVGLISH VLEQNQEGDA AYRKALDLAR EFLPQGPVAM RVAKLAINQG MEVDLVTGLA IEEACYAQTI PTKDRLEGLL AFKEKRPPRY KGE.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Metabolic pathways; Valine, leucine and isoleucine degradation; Metabolism of amino acids and derivativesV

Gene Information

• Gene Name: AUH AU RNA binding protein/enoyl-CoA hydratase [ Homo sapiens ]
• Synonyms: AU RNA binding protein/enoyl-CoA hydratase; AUH; methylglutaconyl-CoA hydratase, mitochondrial; 3-methylglutaconyl-CoA hydratase; AU-binding protein/Enoyl-CoA hydratase; AU-specific RNA-binding enoyl-CoA hydratase; AU RNA binding protein/enoyl-Coenzyme A hydratase; AU RNA-binding protein/enoyl-Coenzyme A hydratase; EC 4.2.1.18
• Gene ID: 549
• mRNA Refseq: NM_001698
• Protein Refseq: NP_001689
• MIM: 600529
• UniProt ID: Q13825
• Chromosome Location: 9q22.31
• Function: RNA binding; enoyl-CoA hydratase activity; lyase activity; mRNA 3"-UTR binding; methylglutaconyl-CoA hydratase activity