Recombinant Human ATP6V1E1, T7-tagged

• Cat.No.: ATP6V1E1-386H
• Product Overview: Recombinant human ATP6V1E1 (aa. 1-226) produced inE.coliis a 26 kDa protein. It was fused to T7 tag at N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Protein Length: 1-226 a.a.
• Description: This protein is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of a hexamer of three A and three B subunits plus the C, D, and E subunits. It contains the ATP catalytic site. The protein is known as the E subunit and is found ubiquitously.
• Purity: 95%.
• Preparation: 0.1mg/0.073 ml.
• Tested Application: E, WB, MS.
• Buffer: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3.
• Species Reactivity: n/aH.
• Storage: Store at -70°C with any protein, exposing ATP6V1E1 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Pathways: Epithelial cell signaling in Helicobacter pylori infection; Metabolic pathways; Oxidative phosphorylation; Vibrio cholerae infection

Gene Information

• Gene Name: ATP6V1E1 ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 [ Homo sapiens ]
• Synonyms: ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1; P31; Vma4; ATP6E; ATP6E2; ATP6V1E; ATP6V1E1; vacuolar H+ ATPase E1; V-ATPase, subunit E; H+-transporting ATP synthase chain E, vacuolar; H(+)-transporting two-sector ATPase, 31kDa subunit; ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31Kd; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1; V-ATPase 31 kDa subunit; V-ATPase subunit E 1; Vacuolar proton pump subunit E 1
• Gene ID: 529
• mRNA Refseq: NM_001039366
• Protein Refseq: NP_001034455
• MIM: 108746
• UniProt ID: P36543
• Chromosome Location: 22q11.1
• Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; hydrolase activity; protein binding; proton-transporting ATPase activity, rotational mechanism