Recombinant Human ATP6V1B2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ATP6V1B2-2300H
• Product Overview: ATP6V1B2 MS Standard C13 and N15-labeled recombinant protein (NP_001684) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.
• Molecular Mass: 56.4 kDa
• AA Sequence: MALRAMRGIVNGAAPELPVPTGGPAVGAQEQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIRTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVGGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ATP6V1B2 ATPase H+ transporting V1 subunit B2 [ Homo sapiens (human) ]
• Official Symbol: ATP6V1B2
• Synonyms: ATP6V1B2; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2; ATP6B2, ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide, 56/58kD, isoform 2, ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2, VPP3; V-type proton ATPase subunit B, brain isoform; HO57; VATB; Vma2; V-ATPase B2 subunit; V-ATPase subunit B 2; vacuolar proton pump subunit B 2; H+ transporting two-sector ATPase; vacuolar H+-ATPase 56,000 subunit; endomembrane proton pump 58 kDa subunit; VPP3; ATP6B2; ATP6B1B2
• Gene ID: 526
• mRNA Refseq: NM_001693
• Protein Refseq: NP_001684
• MIM: 606939
• UniProt ID: P21281