Recombinant Human ATP6V0E1 293 Cell Lysate

• Cat.No.: ATP6V0E1-8586HCL

Specification

• Description: Antigen standard for ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: ATP6V0E1?ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 [Homo sapiens?(human) ]
• Official Symbol: ATP6V0E1
• Synonyms: ATP6V0E1; ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1; M9.2; ATP6H; Vma21; Vma21p; ATP6V0E; V-type proton ATPase subunit e 1; V-ATPase H subunit; V-ATPase subunit e 1; V-ATPase M9.2 subunit; vacuolar proton pump H subunit; vacuolar ATP synthase subunit H; vacuolar proton pump subunit e 1; vacuolar proton-ATPase subunit M9.2; V-ATPase 9.2 kDa membrane accessory protein; H(+)-transporting two-sector ATPase, subunit H; NP_003936.1; EC 3.6.3.14
• Gene ID: 8992
• mRNA Refseq: NM_003945
• Protein Refseq: NP_003936
• MIM: 603931
• UniProt ID: O15342
• Chromosome Location: 5q35.1
• Pathway: Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Epithelial cell signaling in Helicobacter pylori infection, conserved biosystem; Oxidative phosphorylation, organism-specific biosystem
• Function: ATPase activity, coupled to transmembrane movement of ions; proton-transporting ATPase activity, rotational mechanism; transporter activity