Recombinant Human ATP5PD Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ATP5PD-6016H
• Product Overview: ATP5H MS Standard C13 and N15-labeled recombinant protein (NP_006347) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 18.5 kDa
• AA Sequence: MAGRKLALKTIDWVAFAEIIPQNQKAIASSLKSWNETLTSRLAALPENPPAIDWAYYKANVAKAGLVDDFEKKFNALKVPVPEDKYTAQVDAEEKEDVKSCAEWVSLSKARIVEYEKEMEKMKNLIPFDQMTIEDLNEAFPETKLDKKKYPYWPHQPIENLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ATP5PD ATP synthase peripheral stalk subunit d [ Homo sapiens (human) ]
• Official Symbol: ATP5PD
• Synonyms: ATP5PD; ATP synthase peripheral stalk subunit d; ATPQ; APT5H; ATP5H; ATP synthase subunit d, mitochondrial; ATP synthase D chain, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATP synthase, H+ transporting, mitochondrial Fo complex subunit D; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d; ATPase subunit d; My032 protein; EC 3.6.1.14
• Gene ID: 10476
• mRNA Refseq: NM_006356
• Protein Refseq: NP_006347
• MIM: 618121
• UniProt ID: O75947