Recombinant Human ATP5MF Protein, GST/His-tagged

• Cat.No.: ATP5MF-01H
• Product Overview: Purified recombinant protein of Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2 (ATP5J2), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&GST
• Description: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The catalytic portion of mitochondrial ATP synthase consists of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The proton channel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the f subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has multiple pseudogenes. Naturally occurring read-through transcription also exists between this gene and the downstream pentatricopeptide repeat domain 1 (PTCD1) gene.
• Molecular Mass: 38.7 kDa
• Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: > 50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris,pH8.0,150mM NaCl,10 % glycerol,1 % Sarkosyl.
• Full Length: Full L.

Gene Information

• Gene Name: ATP5MF ATP synthase membrane subunit f [ Homo sapiens (human) ]
• Official Symbol: ATP5MF
• Synonyms: ATP5MF; ATP synthase membrane subunit f; ATP5J2; ATP5JL; ATP synthase subunit f, mitochondrial; ATP synthase f chain, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f; ATP synthase, H+ transporting, mitochondrial Fo complex subunit F2; F1F0-type ATPase subunit f; F1Fo-ATP synthase complex Fo membrane domain f subunit; F1Fo-ATPase synthase f subunit; EC 3.6.1.14
• Gene ID: 9551
• mRNA Refseq: NM_004889
• Protein Refseq: NP_004880
• UniProt ID: P56134