Recombinant Human ATP2B3 Protein, His-tagged

• Cat.No.: ATP2B3-267H
• Product Overview: Recombinant Human ATP2B3 Protein (Thr1057-Leu1220) with N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.1 kDa
  Accurate Molecular Mass: 25 kDa
• Protein length: Thr1057-Leu1220
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ATP2B3 ATPase, Ca++ transporting, plasma membrane 3 [ Homo sapiens (human) ]
• Official Symbol: ATP2B3
• Synonyms: ATP2B3; ATPase, Ca++ transporting, plasma membrane 3; plasma membrane calcium-transporting ATPase 3; plasma membrane calcium transporting ATPase 3; PMCA3; plasma membrane calcium pump; plasma membrane calcium ATPase; PMCA3a
• Gene ID: 492
• mRNA Refseq: NM_001001344
• Protein Refseq: NP_001001344
• MIM: 300014
• UniProt ID: Q16720