Recombinant Human ATP, His-tagged

• Cat.No.: ATP6V1A-10040H
• Product Overview: Recombinant Human ATPHis-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Description: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c, c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: 268-617a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: ATP6V1A ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A [ Homo sapiens ]
• Official Symbol: ATP6V1A
• Synonyms: ATP6V1A; ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A; ATP6A1, ATP6V1A1, ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha polypeptide, 70kD, isoform 1 , VPP2; V-type proton ATPase catalytic subunit A; VA68; Vma1; V-ATPase subunit A; V-ATPase A subunit 1; V-ATPase 69 kDa subunit 1; vacuolar ATPase isoform VA68; vacuolar proton pump subunit alpha; vacuolar proton pump alpha subunit 1; ATPase, H+ transporting, lysosomal, subunit A1; H(+)-transporting two-sector ATPase, subunit A; H+-transporting ATPase chain A, vacuolar (VA68 type); vacuolar ATP synthase catalytic subunit A, ubiquitous isoform; HO68; VPP2; ATP6A1; ATP6V1A1
• Gene ID: 523
• mRNA Refseq: NM_001690
• Protein Refseq: NP_001681
• MIM: 607027
• UniProt ID: P38606
• Chromosome Location: 3q13.31
• Pathway: Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Epithelial cell signaling in Helicobacter pylori infection, organism-specific biosystem; Epithelial cell signaling in Helicobacter pylori infection, conserved biosystem; Insulin receptor recycling, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Metabolic pathways, organism-specific biosystem
• Function: ATP binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrolase activity; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; nucleotide binding; proton-transporting ATPase activity