Recombinant Human AST2, His-tagged

• Cat.No.: AST2-3667H
• Product Overview: Aspartate Aminotransferase 2 (AST2)

Specification

• Description: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene.
• Source: E. Coli
• Species: Human
• Tag: His
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 46.2kDa
• Protein length: 387
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of AST2 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: AST2
• Official Symbol: AST2
• Synonyms: GOT2; GOT2; KAT4; KATIV; mitAAT; mAspAT;...
• Gene ID: 2806
• mRNA Refseq: NM_001286220.1
• Protein Refseq: NP_001273149.1
• MIM: 138150
• UniProt ID: P00505
• Chromosome Location: Chromosome: 16; NC_000016.9 (58741035..58768246, complement). Location: 16q21
• Function: ?GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles