Recombinant Human ASH2L Protein, Full Length, N-GST tagged

• Cat.No.: ASH2L-25HFL
• Product Overview: Full-length recombinant human ASH2L was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Description: Enables beta-catenin binding activity and transcription cis-regulatory region binding activity. Contributes to histone methyltransferase activity (H3-K4 specific). Involved in histone H3-K4 methylation; positive regulation of cell population proliferation; and response to estrogen. Acts upstream of or within cellular response to DNA damage stimulus. L centigradeated in nucleus. Part of MLL3/4 complex and Set1C/COMPASS complex.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: Full Length
• Molecular Mass: ~94 kDa
• Purity: > 70%
• Stability: 1 year at –70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Shipping: Dry ice
• References: 1. Patel A, et al: On the mechanism of multiple lysine methyla-tion by the human mixed lineage leukemia protein-1 (MLL1) core complex. J Biol Chem. 2009 284(36):24242-56.
  2. Qi J, et al: Absent, small or homeotic 2-like protein (ASH2L) enhances the transcription of the estrogen receptor α gene through GATA-binding protein 3 (GATA3). J Biol Chem. 2014 289(45):31373-81.

Gene Information

• Gene Name: ASH2L ash2 (absent, small, or homeotic)-like (Drosophila) [ Homo sapiens (human) ]
• Official Symbol: ASH2L
• Synonyms: ASH2L; ash2 (absent, small, or homeotic)-like (Drosophila); ash2 (absent, small, or homeotic, Drosophila, homolog) like , ASH2L1; set1/Ash2 histone methyltransferase complex subunit ASH2; ASH2; ASH2L2; Bre2; ASH2-like protein; ASH2L1
• Gene ID: 9070
• mRNA Refseq: NM_001105214
• Protein Refseq: NP_001098684
• MIM: 604782
• UniProt ID: Q9UBL3