Recombinant Human ARSB protein, His-tagged

• Cat.No.: ARSB-599H
• Product Overview: Recombinant Human ARSB (P15848) aa. (Leu81~Met533) fused with His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Leu81~Met533
• Description: Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 55.2kDa; Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
• Purity: >80%
• Characteristic: Isoelectric point is 7.7
  .
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: ARSB arylsulfatase B [ Homo sapiens (human) ]
• Official Symbol: ARSB
• Synonyms: ARSB; arylsulfatase B; ASB; G4S; MPS6; arylsulfatase B; N-acetylgalactosamine-4-sulfatase
• Gene ID: 411
• mRNA Refseq: NM_000046.3
• Protein Refseq: NP_000037.2
• UniProt ID: P15848