Recombinant Human ARNTL Protein(1-303 aa), His-tagged

• Cat.No.: ARNTL-9879H
• Product Overview: Recombinant Human ARNTL Protein(1-303 aa), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-303 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• AA Sequence: MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESMDTDKDDPHGRLEYTEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFPSTCSKKKDRKSFCTIHSTGYLKSWPPTKMGLDEDNEP
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 μg/μl in 200 μl sterile water for short-term storage.
  After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage.

Gene Information

• Gene Name: ARNTL aryl hydrocarbon receptor nuclear translocator-like [ Homo sapiens ]
• Official Symbol: ARNTL
• Synonyms: ARNTL; aryl hydrocarbon receptor nuclear translocator-like; aryl hydrocarbon receptor nuclear translocator-like protein 1; bHLHe5; BMAL1; JAP3; MOP3; PASD3; bHLH-PAS protein JAP3; member of PAS protein 3; member of PAS superfamily 3; brain and muscle ARNT-like 1; PAS domain-containing protein 3; ARNT-like protein 1, brain and muscle; basic-helix-loop-helix-PAS orphan MOP3; basic-helix-loop-helix-PAS protein MOP3; class E basic helix-loop-helix protein 5; TIC; BMAL1c; MGC47515
• Gene ID: 406
• mRNA Refseq: NM_001030272
• Protein Refseq: NP_001025443
• MIM: 602550
• UniProt ID: O00327

Case Study

Case 1: Kaneko H, et al. Cell Mol Life Sci. 2023

To get a grip on how human-induced pluripotent stem cells (iPSCs) work, especially for things like regenerative medicine, we need to understand their physiology. These stem cells can renew themselves and turn into various cell types, but they don't have some traits of regular cells, like the natural day-night rhythm. Researchers looked into why iPSCs lack this rhythmic behavior. It seems linked to clock genes being turned off due to specific changes in their genetic material or not enough BMAL1 protein. To test this, scientists created cells with extra BMAL1 and treated them with a substance that affects gene repression.

Fig1. BMAL1 and CLOCK protein levels were measured in U2OS cells, 201B7 iPSCs, and 201B7-BMAL1.

Fig2. Expression levels of the clock genes were measured in 201B7-BMAL1 cells.

Case 2: Xu H, et al. Int Endod J. 2022

We explored how Bmal1 impacts dental pulp stem cells (DPSCs) under inflammation. Using cells from healthy donors, we added different doses of LPS to mimic this stress and checked changes in genes like Bmal1 with real-time PCR. Western blot helped us see protein expressions that relate to circadian clocks and dentinogenic differentiation. We also checked how these cells responded in terms of cell death and differentiation under inflammation. Notably, in a low-LPS setup, BMAL1 levels rose, boosting dentinogenic differentiation. But with more LPS, BMAL1 levels dropped, hampering differentiation. Our findings suggest BMAL1 might influence this process through the PI3K/Akt/mTOR pathway.

Fig1. Western blot for BMAL1 and CLOCK on different concentrations of LPS-stimulation.

Fig2. Western blot analysis of dentinogenic differentiation related proteins DMP-1, DSPP and COL-1 in Bmal1-knockout DPSCs.

Application

Recombinant Human ARNTL (rhARNTL) plays a big role in research, especially when it comes to understanding circadian rhythms and cell function. ARNTL is a key clock gene in the bHLH-PAS transcription factor family, and it's crucial for regulating our body's natural rhythms. Scientists look into rhARNTL to see how it affects things like circadian rhythms, cell growth, and even immune responses. For example, some studies have shown that ARNTL is linked to the proportion of mesenchymal cells in carotid plaques. When researchers reduced ARNTL levels in lab settings, they noticed an increase in contraction markers and a drop in cell proliferation in human carotid smooth muscle cells.

In the industrial world, rhARNTL shows up in drug development and biopharmaceuticals. Given ARNTL's key role across different biological processes, it's emerging as a promising drug target for crafting treatments that can adjust circadian rhythms and cellular functions. Additionally, rhARNTL is employed in the production of antibodies for research, which are essential in a variety of experimental techniques such as immunohistochemistry, Western Blot, immunocytochemistry, ELISA, and flow cytometry. These uses not only deepen our understanding of ARNTL's biological roles but also open doors to developing innovative therapies.

Fig1. New insights into the mechanisms of diabetic kidney disease: Role of circadian rhythm and Bmal1. (Zhimei Peng, 2023)