Recombinant Human ARHGDIB, GST-tagged

• Cat.No.: ARHGDIB-29H
• Product Overview: Recombinant human ARHGDIB protein, fused with GST-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases.
• Source: E.Coli.
• Species: Human
• Tag: GST
• Form: Liquid. In phosphate-buffered Saline(pH 7.4) containing 10% glycerol
• Molecular Mass: 49.4 kDa (428aa)
• Protein length: 1-201aa
• AA Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERAEISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHSLIKYKKTLL GDGPVVTDPK APNVVVTRLT LVCESAPGPI TMDLTGDLEA LKKETIVLKE GSEYRVKIHF KVNRDIVSGL KYVQHTYRTGMTE KAPEPHVEED DDDELDSKLN YKPPPQKSLK ELQEMDKDDEVKVDKATFMV GSYGPRPEEY EFLTPVEEAP KGMLARGTYH NKSFFTDDDK QDHLSWEWNL SIKKEWTE
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ARHGDIB Rho GDP dissociation inhibitor (GDI) beta [ Homo sapiens (human) ]
• Official Symbol: ARHGDIB
• Synonyms: ARHGDIB; Rho GDP dissociation inhibitor (GDI) beta; GDIA2, GDID4, RAP1GN1; rho GDP-dissociation inhibitor 2; Ly GDI; RhoGDI2; Rho GDI 2; rho-GDI beta; D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1
• Gene ID: 397
• mRNA Refseq: NM_001175
• Protein Refseq: NP_001166
• MIM: 602843
• UniProt ID: P52566
• Chromosome Location: 12p12.3
• Pathway: Caspase cascade in apoptosis; FAS pathway and Stress induction of HSP regulation; Neurotrophin signaling pathway
• Function: GTPase activator activity; Rho GDP-dissociation inhibitor activity