Recombinant Human ARAF protein, GST-tagged

• Cat.No.: ARAF-32H
• Product Overview: Recombinant Human ARAF(209-end) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Description: ARAF belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. ARAF play a critical role in cell growth and development (1).The N-terminal regulatory domain of ARAF interacted with the putative mitochondrial proteins TOM and TIM44. ARAF is expressed predominantly in urogenital tissues (2). The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus.
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~70 kDa
• Purity: >70%
• Applications: Western Blot
• Stability: 1 year from date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl
• Protein length: 209-end a.a.

Gene Information

• Gene Name: ARAF v-raf murine sarcoma 3611 viral oncogene homolog [ Homo sapiens ]
• Official Symbol: ARAF
• Synonyms: ARAF; v-raf murine sarcoma 3611 viral oncogene homolog; ARAF1, v raf murine sarcoma 3611 viral oncogene homolog 1; serine/threonine-protein kinase A-Raf; Oncogene ARAF1; proto-oncogene Pks; proto-oncogene A-Raf-1; Ras-binding protein DA-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; A-Raf proto-oncogene serine/threonine-protein kinase; PKS2; A-RAF; ARAF1; RAFA1
• Gene ID: 369
• mRNA Refseq: NM_001654
• Protein Refseq: NP_001645
• MIM: 311010
• UniProt ID: P10398
• Chromosome Location: Xp11.3-p11.23
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Chronic myeloid leukemia, organism-specific biosystem; Chronic myeloid leukemia, conserved biosystem; Colorectal cancer, organism-specific biosystem
• Function: ATP binding; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; receptor signaling protein activity