Recombinant Human AR, His-tagged

• Cat.No.: AR-1012H
• Product Overview: Recombinant Human Androgen Receptor Ligand Binding Domain (650-920) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 650-920 a.a.
• Description: The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.
• Form: The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
• Molecular Mass: 33.7 kDa.
• AA Sequence: TSPTEETTQK LTVSHIEGYE CQPIFLNVLE AIEPGVVCAG HDNNQPDSFA ALLSSLNELG ERQLVHVVKW AKALPGFRNL HVDDQMAVIQ YSWMGLMVFA MGWRSFTNVN SRMLYFAPDLVFNEYRMHKS RMYSQCVRMR HLSQEFGWLQ ITPQEFLCMK ALLLFSIIPV DGLKNQKFFD ELRMNYIKEL DRIIACKRKN PTSCSRRFYQ LTKLLDSVQP IARELHQFTF DLLIKSHMVSVDFPEMMAEI ISVQVPKILS GKVKPIYFHT Q
• Purity: The purified AR-LBD is greater than 90% homogeneous based on SDS-PAGE analysis.
• Unit Definition: 1 unit equals 1 nanogram of purified protein.
• Applications: AR can be used for gel mobility shift assays, for protein-protein and small molecules-protein interactions assay.
• Storage: Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Gene Information

• Gene Name: AR androgen receptor [ Homo sapiens (human) ]
• Official Symbol: AR
• Synonyms: AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; androgen receptor; dihydrotestosterone receptor; androgen nuclear receptor variant 2; nuclear receptor subfamily 3 group C member 4
• Gene ID: 367
• mRNA Refseq: NM_000044
• Protein Refseq: NP_000035
• MIM: 313700
• UniProt ID: P10275
• Chromosome Location: Xq12
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway; Coregulation of Androgen receptor activity; FOXA1 transcription factor network
• Function: DNA binding; RNA polymerase II transcription factor binding; androgen binding