Recombinant Human AQP9 293 Cell Lysate

• Cat.No.: AQP9-8765HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for aquaporin 9 (AQP9) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AQP9 aquaporin 9 [ Homo sapiens ]
• Official Symbol: AQP9
• Synonyms: AQP9; aquaporin 9; aquaporin-9; HsT17287; SSC1; aquaglyceroporin-9; small solute channel 1; AQP-9
• Gene ID: 366
• mRNA Refseq: NM_020980
• Protein Refseq: NP_066190
• MIM: 602914
• UniProt ID: O43315
• Chromosome Location: 15q
• Pathway: Aquaporin-mediated transport, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Passive Transport by Aquaporins, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem; Transport of Glycerol from Adipocytes to the Liver by Aquaporins, organism-specific biosystem
• Function: amine transmembrane transporter activity; carboxylic acid transmembrane transporter activity; glycerol channel activity; polyol transmembrane transporter activity; porin activity; purine base transmembrane transporter activity; pyrimidine base transmembrane transporter activity; transporter activity; water channel activity; water transmembrane transporter activity