Recombinant Human Apolipoprotein A-I, His-tagged

• Cat.No.: APOA1-6916H
• Product Overview: Recombinant human APOA1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 30.3 kDa.

Specification

• Description: APOA1 is a human protein with a specific role in lipid metabolism. It is the major protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion. And it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the ApoA-1 gene are associated with HDL deficiency including Tangier disease.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing, 10% glycerol.
• Molecular Mass: 30.3 kDa (264aa), confirmed by MALDI-TOF
• Protein length: 25-267aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ
• Purity: >95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: APOA1 apolipoprotein A-I [ Homo sapiens ]
• Official Symbol: APOA1
• Synonyms: APOA1; apolipoprotein A-I; apo-AI; apoA-I; MGC117399
• Gene ID: 335
• mRNA Refseq: NM_000039
• Protein Refseq: NP_000030
• MIM: 107680
• UniProt ID: P02647
• Chromosome Location: 11q23-q24
• Pathway: ABC-family proteins mediated transport, organism-specific biosystem; ABCA transporters in lipid homeostasis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Amyloids, organism-specific biosystem; Chylomicron-mediated lipid transport, organism-specific biosystem; Disease, organism-specific biosystem
• Function: apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; cholesterol binding; contributes_to cholesterol transporter activity; cholesterol transporter activity; enzyme binding; high-density lipoprotein particle binding; high-density lipoprotein particle receptor binding; identical protein binding; lipid transporter activity; phosphatidylcholine-sterol O-acyltransferase activator activity; phospholipid binding; protein binding