Recombinant Human APOD, His-tagged

• Cat.No.: APOD-3653H
• Product Overview: Recombinant Human APOD, fused with His-tag (7 extra AA) at C-terminal, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism.?
• Form: Filtered (0.4?μm) and lyophilized from 1.0?mg/mL in 4?mM KH2PO4; 16?mM Na2HPO4; 115?mM NaCl; pH?7.5
• Molecular Mass: 19.82?kDa (calculated)
• AA Sequence: FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHH HHHH
• Endotoxin: <1.0?eu>
• Purity: >95%
• Applications: ELISA, Western blotting
• Quality Control Test: BCA to determine content of the proteinSDS-PAGE to determine purity of the proteinLAL TEST to determine quantity of the endotoxin.
• Notes: This product is intended for research use only.
• Storage: Store lyophilized protein at ?20°C. Lyophilized protein remains stable until the expiry date when stored at ?20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
• Reconstitution: Add deionized water to a working concentration approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter this product by an appropriate sterile filter before using it in the cell culture.
• Shipping: At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Gene Information

• Gene Name: APOD apolipoprotein D [ Homo sapiens ]
• Official Symbol: APOD
• Synonyms: APOD; apolipoprotein D; apo-D
• Gene ID: 347
• mRNA Refseq: NM_001647
• Protein Refseq: NP_001638
• MIM: 107740
• UniProt ID: P05090
• Chromosome Location: 3q29
• Function: cholesterol binding; lipid transporter activity; protein binding