Recombinant human APOA5, His-tagged

• Cat.No.: APOA5-2476H
• Product Overview: Recombinant human APOA5 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Description: APOA5 is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
• Molecular Mass: 41.3kDa (366aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSRKGFWDY FSQTSGDKGR VEQIHQQKMA REPATLKDSL EQDLNNMNKF LEKLRPLSGS EAPRLPQDPVGMRRQLQEEL EEVKARLQPY MAEAHELVGW NLEGLRQQLK PYTMDLMEQV ALRVQELQEQ LRVVGEDTKA QLLGGVDEAW ALLQGLQSRV VHHTGRFKEL FHPYAESLVS GIGRHVQELH RSVAPHAPAS PARLSRCVQV LSRKLTLKAK ALHARIQQNL DQLREELSRA FAGTGTEEGAGPDPQMLSEE VRQRLQAFRQ DTYLQIAAFT RAIDQETEEV QQQLAPPPPG HSAFAPEFQQ TDSGKVLSKL QARLDDLWED ITHSLHDQGH SHLGDP
• Purity: >85 % by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: APOA5 apolipoprotein A-V [ Homo sapiens ]
• Official Symbol: APOA5
• Synonyms: APOA5; apolipoprotein A-V; APOA V; RAP3; apo-AV; apolipoprotein A5; regeneration-associated protein 3; APOAV; FLJ97995; MGC126836; MGC126838
• Gene ID: 116519
• mRNA Refseq: NM_001166598
• Protein Refseq: NP_001160070
• MIM: 606368
• UniProt ID: Q6Q788
• Chromosome Location: 11q23
• Pathway: Chylomicron-mediated lipid transport, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPAR signaling pathway, organism-specific biosystem
• Function: enzyme activator activity; enzyme binding; heparin binding; contributes_to heparin binding; lipase activator activity; lipid binding; lipoprotein lipase activator activity; lipoprotein particle receptor binding; low-density lipoprotein particle receptor b