Recombinant Human APAF1 protein, His & T7-tagged

• Cat.No.: APAF1-1442H
• Product Overview: Recombinant Human APAF1 aa. (Ser31~Phe350 (Accession # O14727)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 39.6kDa.
• Protein length: Ser31~Phe350
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: APAF1 apoptotic peptidase activating factor 1 [ Homo sapiens (human) ]
• Official Symbol: APAF1
• Synonyms: CED4; APAF-1; apoptotic protease-activating factor 1
• Gene ID: 317
• mRNA Refseq: NM_001160.2
• Protein Refseq: NP_001151.1
• UniProt ID: O14727