Recombinant Human Ankyrin Repeat Domain 1 (Cardiac Muscle), His-tagged

• Cat.No.: ANKRD1-7150H
• Product Overview: Recombinant human ANKRD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 38.6 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-319aa
• Description: Ankyrin repeat domain-containing protein 1, also known as ANKRD1, may play an important role in endothelial cell activation. This protein may act as a nuclear transcription factor that negatively regulates the expression of cardiac genes.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 5mM DTT
• Molecular Mass: 38.6 kDa (342aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMMVLKVE ELVTGKKNGN GEAGEFLPED FRDGEYEAAV TLEKQEDLKT LLAHPVTLGE QQWKSEKQRE AELKKKKLEQ RSKLENLEDL EIIIQLKKRK KYRKTKVPVV KEPEPEIITE PVDVPTFLKA ALENKLPVVE KFLSDKNNPD VCDEYKRTAL HRACLEGHLA IVEKLMEAGA QIEFRDMLES TAIHWASRGG NLDVLKLLLN KGAKISARDK LLSTALHVAV RTGHYECAEH LIACEADLNA KDREGDTPLH DAVRLNRYKM IRLLIMYGAD LNIKNCAGKT PMDLVLHWQN GTKAIFDSLR ENSYKTSRIA TF
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ANKRD1 ankyrin repeat domain 1 (cardiac muscle) [ Homo sapiens ]
• Official Symbol: ANKRD1
• Synonyms: ANKRD1; ankyrin repeat domain 1 (cardiac muscle); ankyrin repeat domain-containing protein 1; ALRP; C 193; CARP; CVARP; MCARP; liver ankyrin repeat domain 1; cardiac ankyrin repeat protein; cytokine-inducible nuclear protein; cytokine-inducible gene C-193 protein; C-193; bA320F15.2
• Gene ID: 27063
• mRNA Refseq: NM_014391
• Protein Refseq: NP_055206
• MIM: 609599
• UniProt ID: Q15327
• Chromosome Location: 10q23.33
• Pathway: Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Hypertrophy Model, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem; Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystem
• Function: DNA binding; R-SMAD binding; RNA polymerase II transcription coactivator activity; RNA polymerase II transcription factor binding; histone deacetylase binding; p53 binding; protein binding; titin binding; transcription corepressor activity