Recombinant Human AMN1 protein, His-tagged

• Cat.No.: AMN1-7854H
• Product Overview: Recombinant Human AMN1 protein(1-213 aa), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: 1-213 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization.
• AASequence: MQGQITDSNISEILHPEVQTLDLRS CDISDAALLHLSNCRKLKKLNLNAS KGNRVSVTSEGIKAVASSCSYLHEA SLKRCCNLTDEGVVALALNCQLLKI IDLGGCLSITDVSLHALGKNCPFLQ CVDFSATQVSDSGVIALVSGPCAKK LEEIHMGHCVNLTDGAVEAVLTYCP QIRILLFHGCPLITDHSREVLEQLV GPNKLKQVTWTVY
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks). Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 μg/μl in 200 μl sterile water for short-term storage. After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage.

Gene Information

• Gene Name: AMN1 antagonist of mitotic exit network 1 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: AMN1
• Synonyms: AMN1; antagonist of mitotic exit network 1 homolog (S. cerevisiae); protein AMN1 homolog
• Gene ID: 196394
• mRNA Refseq: NM_001113402
• Protein Refseq: NP_001106873
• UniProt ID: Q8IY45

Reviews

04/08/2021

The prompt and knowledgeable assistance they offer has greatly enhanced my confidence in using the AMN1 protein.

11/28/2018

Its purity and reliability have provided consistent and reliable results in my research.

09/07/2018

I am highly satisfied with the performance and support associated with this protein, as it has proven to be an essential tool in advancing my scientific investigations.

07/22/2016

The AMN1 protein is of exceptional quality, making it a perfect fit for my experimental needs.

Q&As

Are there any known interactors or regulators of the AMN1 protein?

Several interactors and regulators of the AMN1 protein have been identified through experimental studies and protein-protein interaction databases. These include other ribosomal proteins, chaperones, and factors involved in ribosome biogenesis and translation.

Has the AMN1 protein been linked to any specific biological processes or pathways?

The AMN1 protein is primarily associated with ribosome biogenesis and translation initiation, which are essential for protein synthesis. Dysregulation of these processes can impact cell growth, development, and disease progression.

Does the AMN1 protein have any known protein-protein interactions?

Yes, the AMN1 protein is known to interact with other ribosomal proteins, such as Rps3p and Rps21p, to regulate ribosome assembly and function.

Does the AMN1 protein localize to specific cellular compartments?

The AMN1 protein is primarily localized to the nucleus, where it plays a role in ribosome biogenesis. However, it may also localize to other cellular compartments, such as the nucleolus and cytoplasm, during certain cellular processes or under specific conditions.

Is the expression of the AMN1 gene regulated?

The expression of the AMN1 gene can be regulated by various factors, including environmental signals, cellular processes, and transcription factors. However, the specific regulatory mechanisms of AMN1 gene expression are not fully understood.

Has the AMN1 protein been implicated in any diseases or conditions?

While there are no known diseases directly linked to AMN1 protein mutations, dysregulation of ribosome biogenesis and translation processes can have broad impacts on cell growth, development, and disease progression. Further research may provide insights into potential disease associations.

Are there any known post-translational modifications of the AMN1 protein?

Currently, there is limited information available regarding post-translational modifications of the AMN1 protein. Further research is required to identify and characterize potential modifications such as phosphorylation or ubiquitination.

What are some potential future directions for studying the AMN1 protein?

Future studies can focus on elucidating the structural properties of AMN1 and its exact mechanisms in ribosome biogenesis and translation regulation. Additionally, investigating potential gene regulatory networks involving AMN1 could provide further insights into its biological functions.

Is the AMN1 protein conserved across different species?

The AMN1 protein is evolutionarily conserved, meaning that it is found in various species and shares certain structural and functional features across those species.

Are there any genetic disorders or diseases associated with mutations in the AMN1 gene?

Currently, there are no known genetic disorders or diseases associated with AMN1 gene mutations in humans.

Is there any ongoing research or studies on the AMN1 protein?

While research on the AMN1 protein is relatively limited, ongoing studies continue to investigate its role in ribosome biogenesis and its potential implications in cellular processes.

In which cellular compartment is the AMN1 protein located?

The AMN1 protein is primarily localized in the cytoplasm of cells.

Are there any known mouse models or knockout studies of the AMN1 gene?

Currently, there are no known specific mouse models or knockout studies targeting the AMN1 gene. However, studies involving other ribosomal proteins or factors related to ribosome biogenesis may indirectly provide insights into the function of the AMN1 gene.

Are there any known biological functions of the AMN1 protein apart from ribosome biogenesis?

While the primary known function of the AMN1 protein is in ribosome biogenesis, it may also have additional roles in cellular processes such as mRNA translation, stress responses, and cell cycle regulation. Further research is needed to uncover these potential functions.

Is the AMN1 protein involved in any specific cellular pathways?

The AMN1 protein is mainly associated with ribosome biogenesis and translation initiation. It plays a role in the assembly and maturation of ribosomes, which are essential for protein synthesis.

Can the AMN1 protein interact with other proteins?

Yes, the AMN1 protein can interact with other proteins as part of ribosome assembly and translation initiation processes. It has been shown to physically interact with various ribosomal proteins, assembly factors, and translation initiation factors.

Are there any homologs or orthologs of the AMN1 protein in other species?

Yes, AMN1 protein orthologs have been identified in various eukaryotic organisms, including humans, mice, flies, and plants.

Are there any genetic variants or mutations in the AMN1 gene?

Genetic variants and mutations in the AMN1 gene have been identified in studies and databases. These variants can range from single nucleotide polymorphisms (SNPs) to larger structural variations. Some of these variants may affect the expression or function of the AMN1 protein.

Is the expression of the AMN1 gene tissue-specific?

The expression of the AMN1 gene has been detected in multiple tissues and cell types, suggesting a widespread expression pattern. However, the levels of AMN1 expression may vary across tissues, indicating some degree of tissue specificity.