Recombinant Human AMELX Protein, (AFPn)-His-TEV-tagged

• Cat.No.: AMELX-015H
• Product Overview: Full-length mature human AMELX cDNA (17–191aa, isoforms-1, derived from BC064379) was constructed with codon optimization and expressed with a human Alpha-Fetal Protein N-terminal domain (AFPn)-His-TEV cleavage site (219aa) fusion at its N-terminal.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 17–191
• Description: This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms.
• AA Sequence: MTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSGGSHHHHHHGSENLYFQGEFEMPLPPHPGHPGYINFSYEVLTPLKWYQSIRPPYPSYGYEPMGGWLHHQIIPVLSQQHPPTHTLQPHHHIPVVPAQQPVIPQQPMMPVPGQHSMTPIQHHQPNLPPPAQQPYQPQPVQPQPHQPMQPQPPVHPMQPLPPQPPLPPMFPMQPLPPMLPDLTLEAWPSTDKTKREEVD
• Purity: > 90% by SDS-PAGE.
• Applications: 1. May be used for in vitro AMELX mediated hBMSCx osteogenic differentiation regulation study with this protein as either coating matrix protein or soluble factor.
  2. May be used for AMELX protein-protein interaction assay.
  3. As antigen for specific antibody production.
• Storage: In Liquid. Keep at -80 centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.
• Concentration: 0.5 mg/mL
• Storage Buffer: Sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, Sucrose and DTT.

Gene Information

• Gene Name: AMELX amelogenin X-linked [ Homo sapiens (human) ]
• Official Symbol: AMELX
• Synonyms: AMELX; amelogenin X-linked; AMG; AI1E; AIH1; ALGN; AMGL; AMGX; amelogenin, X isoform; amelogenin (X chromosome, amelogenesis imperfecta 1); amelogenin (amelogenesis imperfecta 1, X-linked)
• Gene ID: 265
• mRNA Refseq: NM_001142
• Protein Refseq: NP_001133
• MIM: 300391
• UniProt ID: Q99217