Recombinant Human Alpha-1-Microglobulin/Bikunin Precursor, His-tagged

• Cat.No.: AMBP-6894H
• Product Overview: Recombinant human AMBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 23.1 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 20-203aa
• Description: AMBP is an immunomodulatory protein with a broad spectrum of possible clinical applications and seems a promising marker for evaluation of tubular function. AMBP specifically interacts with the ORF3 protein of hepatitis E virus and has a direct role in enhancing alpha-1 microglobulin export from the hepatocyte.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
• Molecular Mass: 23.1 kDa (205aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGPVPTPPDN IQVQENFNIS RIYGKWYNLA IGSTCPWLKK IMDRMTVSTL VLGEGATEAE ISMTSTRWRK GVCEETSGAY EKTDTDGKFL YHKSKWNITM ESYVVHTNYD EYAIFLTKKF SRHHGPTITA KLYGRAPQLR ETLLQDFRVV AQGVGIPEDS IFTMADRGEC VPGEQEPEPI LIPRV
• Purity: >90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: AMBP alpha-1-microglobulin/bikunin precursor [ Homo sapiens ]
• Official Symbol: AMBP
• Synonyms: AMBP; alpha-1-microglobulin/bikunin precursor; ITI, ITIL; protein AMBP; bikunin; complex forming glycoprotein heterogeneous in charge; EDC1; growth inhibiting protein 19; HCP; HI30; IATIL; inter alpha trypsin inhibitor light chain; ITILC; protein HC; trypstatin; uristatin; uronic acid rich protein; UTI; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge; A1M; ITI; ITIL
• Gene ID: 259
• mRNA Refseq: NM_001633
• Protein Refseq: NP_001624
• MIM: 176870
• UniProt ID: P02760
• Chromosome Location: 9q32-q33
• Function: IgA binding; calcium channel inhibitor activity; calcium oxalate binding; heme binding; peptidase inhibitor activity; protein homodimerization activity; serine-type endopeptidase inhibitor activity; transporter activity