Recombinant Human Aldo-Keto Reductase Family 1, Member D1 (Delta 4-3-Ketosteroid-5-Beta-Reductase), His-tagged

• Cat.No.: AKR1D1-1674H
• Product Overview: Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Aldo-keto reductase family 1, member D1, also known as AKR1D1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones which carry a delta (4)-3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction.
• Concentration: 0.5 mg/ml
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 20% glycerol and 100mM NaCl.
• Purity: > 95% by SDS - PAGE
• Sequence: 1-326 amino acids: MGSSHHHHHH SSGLVPRGSH MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQV
• Molecular Mass: 39.5 kDa (346aa) confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Pathay: Bile acid and bile salt metabolism; Bile acid biosynthesis, cholesterol => chenodeoxycholate; Bile acid biosynthesis, cholesterol => cholate; Metabolic pathways; Metabolism of lipids and lipoproteins; Primary bile acid biosynthesis; Steroid hormone biosynthesis; Synthesis of bile acids and bile salts; Synthesis of bile acids and bile salts via 24-hydroxycholesterol; Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol

Gene Information

• Gene Name: AKR1D1 aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) [Homo sapiens]
• Official Symbol: AKR1D1
• Synonyms: AKR1D1; aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase); CBAS2; SRD5B1; 3o5bred; 3-oxo-5-beta-steroid 4-dehydrogenase; OTTHUMP00000208643; OTTHUMP00000208644; OTTHUMP00000208645; delta(4)-3-oxosteroid 5-beta-reductase; delta(4)-3-ketosteroid 5-beta-reductase; steroid-5-beta-reductase, beta polypeptide 1 (3-oxo-5 beta-steroid delta 4-dehydrogenase beta 1); EC1.3.1.3; aldo-keto reductase family 1 member D1
• Gene ID: 6718
• mRNA Refseq: NM_005989
• Protein Refseq: NP_005980
• MIM: 604741
• UniProt ID: P51857
• Chromosome Location: 7q32-q33
• Function: 3-oxo-5-beta-steroid 4-dehydrogenase activity; aldo-keto reductase (NADP) activity; delta4-3-oxosteroid 5beta-reductase activity; oxidoreductase activity; steroid binding