Recombinant Human Alcohol Dehydrogenase 1A (class I), Alpha Polypeptide, His-tagged

• Cat.No.: ADH1A-1917H
• Product Overview: Recombinant humanADH1A protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Cat. No.: ADH1A-1917H
• Description: ADH1Abelongs to the alcohol dehydrogenase family. ADH1A is a monomorphic andpredominant in fetal and infant livers, becoming less active in gestation andonly weakly active during adulthood. ADH1A plays a major role in ethanolmetabolism. With the coenzyme NAD, ADH catalyzes the reversible conversion oforganic alcohols to ketones or aldehydes.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
• Molecular Weight: 42 kDa(395aa) confirmed by MALDI-TOF
• Purity: > 90%by SDS-PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MSTAGKVIKC KAAVLWELKK PFSIEEVEVA PPKAHEVRIK MVAVGICGTD DHVVSGTMVTPLPVILGHEAAGIVESVGEGVTTVKPGDKV IPLAIPQCGK CRICKNPESN YCLKNDVSNP QGTLQDGTSR FTCRRKPIHH FLGISTFSQYTVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG LGGVGLSAIM GCKAAGAARIIAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTDGGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSMNPMLLL TGRTWKGAIL GGFKSKECVPKLVADFMAKK FSLDALITHV LPFEKINEGF DLLHSGKSIR TILMF
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Biological oxida; Drug metabolism-cytochromeP450; Ethanol oxidation; Fatty Acid Omega Oxidation; Fatty acid metabo;Glycolysis / Gluconeogenesis; Metabolic pathways; Metabolism; Metabolism ofxenobiotics by cytochrome P450; Phase 1-Functionalization of compounds;Retinol metabolism; Tyrosine metabolism; Vitamin A and carotenoid metabolism

Gene Information

• Gene Name: ADH1A alcohol dehydrogenase 1A(class I), alpha polypeptide [ Homo sapiens ]
• Official Symbol: ADH1A
• Synonyms: ADH1A; alcohol dehydrogenase 1A (class I),alpha polypeptide; ADH1; alcohol dehydrogenase 1A; ADH, alphasubunit; aldehyde reductase; alcohol dehydrogenase subunit alpha; alcoholdehydrogenase 1 (class I), alpha polypeptide; EC 1.1.1.1; Alcohol dehydrogenase 1A
• Gene ID: 124
• mRNA Refseq: NM_000667
• Protein Refseq: NP_000658
• MIM: 103700
• UniProt ID: P07327
• Chromosome Location: 4q23
• Function: alcohol dehydrogenase activity; metal ionbinding; nucleotide binding; oxidoreductase activity; protein binding; zincion binding