Recombinant Human AKT3, His-tagged

• Cat.No.: AKT3-166H
• Product Overview: Recombinant Human AKT3 was expressed in E. coli. cells containing 6His tag at N-terminus and is purified by Ni-NTA agarose, 66kDa (47aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
• Purity: ~50%
• amino acid sequence: MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMAISDPDSATMSDVTIVKEGWVQKRGEYIKNWRPRYFLLKTDGSFIGYKEKPQDVDLPYPLNNFSVAKCQLMKTERPKPNTFIIRCLQWTTVIERTFHVDTPEEREEWTEAIQAVADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFDYLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDEVAHTLTESRVLKNTRHPFLTSLKYSFQTKDRLCFVMEYVNGGELFFHLSRERVFSEDRTRFYGAEIVSALDYLHSGKIVYRDLKLENLMLDKDGHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKYDEDGMDCMDNERRPHFPQFSYSASGRE
• Formulation: 15µg of recombinant protein in 100µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1% (v/v) 2-mercaptoethanol, 0.1mM EGTA, 1mM benzamidine, and 20% glycerol. Frozen solution.
• Storage andStability: Stable for 6 months at -20°C.from date of shipment. For maximum yield, centrifuge the vial after thawing and prior to removing the cap.

Gene Information

• Gene Name: AKT3 v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) [ Homo sapiens ]
• Official Symbol: AKT3
• Synonyms: AKT3; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); PKBG; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma; DKFZp434N0250; RAC-gamma serine/threonine-protein kinase; PKB gamma; EC 2.7.11.1; Protein kinase Akt-3; Protein kinase B gamma; OTTHUMP00000037911; OTTHUMP00000037912; serine threonine protein kinase, Akt-3; EC 2.7.11
• Gene ID: 10000
• mRNA Refseq: NM_001206729
• Protein Refseq: NP_001193658
• MIM: 611223
• UniProt ID: Q9Y243
• Chromosome Location: 1q44
• Pathway: Acute myeloid leukemia; Adaptive Immune System; Apoptosis
• Function: ATP binding; nucleotide binding; protein binding