Recombinant Human AK4, His-tagged

• Cat.No.: AK4-3448H
• Product Overview: Recombinant human AK4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: AK4-3448H
• Description: AK4(adenylate kinase 4) is a member of the adenylate kinase family of enzymes. This protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Inherited mutations leading to AK4 deficiencies in erythrocytes have been implicated in hemolytic anemia.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Molecular Weight: 27.4 kDa (243aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• Purity: > 90 % by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: AK4 adenylate kinase 4 [ Homo sapiens ]
• Synonyms: AK3L1; adenylate kinase 3-like 1; AK3L1 adenylate kinase 3-like 1; AK3; AK4; MGC166959; EC 2.7.4.3; OTTHUMP00000010594; ATP-AMP transphosphorylase; GTP:AMP phosphotransferase; Adenylate kinase 3-like; adenylate kinase 3; mitochondrial adenylate kinase-3; nucleoside-triphosphate-adenylate kinase
• Gene ID: 205
• mRNA Refseq: NM_001005353
• Protein Refseq: NP_001005353
• MIM: 103030
• UniProt ID: P27144
• Chromosome Location: 1p31.3
• Pathway: Purine metabolism
• Function: ATP binding; GTP binding; adenylate kinase activity; nucleotide binding; transferase activity