Recombinant Human AIDA, His-tagged

• Cat.No.: AIDA-7533H
• Product Overview: Recombinant human AIDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-306 aa
• Antigen Description: AIDA acts as a ventralizing factor during embryogenesis. This protein inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.2M NaCl,1mM DTT
• Molecular Mass: 37.4kDa(329aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSEVTRS LLQRWGASFR RGADFDSWGQ LVEAIDEYQI LARHLQKEAQ AQHNNSEFTE EQKKTIGKIA TCLELRSAAL QSTQSQEEFK LEDLKKLEPI LKNILTYNKE FPFDVQPVPL RRILAPGEEE NLEFEEDEEE GGAGAGSPDS FPARVPGTLL PRLPSEPGMT LLTIRIEKIG LKDAGQCIDP YITVSVKDLN GIDLTPVQDT PVASRKEDTY VHFNVDIELQ KHVEKLTKGA AIFFEFKHYK PKKRFTSTKC FAFMEMDEIK PGPIVIELYK KPTDFKRKKL QLLTKKPLYL HLHQTLHKE
• Purity: >90% as determined by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml

Gene Information

• Gene Name: AIDA axin interactor, dorsalization associated [ Homo sapiens ]
• Official Symbol: AIDA
• Synonyms: AIDA; axin interactor, dorsalization associated; C1orf80, chromosome 1 open reading frame 80; axin interactor, dorsalization-associated protein; axin interaction partner and dorsalization antagonist; FLJ12806; C1orf80; FLJ32421; RP11-378J18.7
• Gene ID: 64853
• mRNA Refseq: NM_022831
• Protein Refseq: NP_073742
• MIM: 612375
• UniProt ID: Q96BJ3
• Chromosome Location: 1q41
• Function: protein domain specific binding