Recombinant Human AFDN Protein

• Cat.No.: AFDN-820H
• Product Overview: Recombinant Human AFDN (residues 1-141) was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-141 a.a.
• Description: The amino terminus of Af6 was identified as the Rasbinding site. Additionally to the Ras-binding Af6 also binds Rap. ZO-1, a protein involved in the formation of tight junctions, also binds to Af6 close to the amino terminus thereby competing with Ras binding. These data suggest a participation of Af6 in the regulation of cell-cell contacts via a Ras-modulated interaction with ZO-1. In contrast to the well characterized effectors Raf, RalGEF, and PI(3)kinase, Af6 is a protein with no enzymatic function. It seems to serve as a scaffolding component for protein complexes. This would represent a new type of Ras effector because Ras binding in this case does not lead to the activation of the enzymatic activity of the effector.
• Form: 25 mM HEPES pH 7.5, 50 mM NaCl, 1 mM EDTA and 50% glycerol
• Purity: > 95 % (SDS-PAGE)
• Storage: Store at -20 centigrade, avoid freeze/thaw cycles.

Gene Information

• Gene Name: AFDN afadin, adherens junction formation factor [ Homo sapiens (human) ]
• Official Symbol: AFDN
• Synonyms: AF6; MLLT4; MLL-AF6; l-afadin
• Gene ID: 4301
• mRNA Refseq: NM_001207008.1
• Protein Refseq: NP_001193937.1
• MIM: 159559
• UniProt ID: P55196