Recombinant Human ADP-Ribosylation Factor 3, His-tagged

• Cat.No.: ARF3-6867H
• Product Overview: Recombinant human ARF3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 22.8 kDa.

Specification

• Description: ARF3 functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. This protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT.
• Molecular Mass: 22.8 kDa (201aa), confirmed by MALDI-TOF.
• Protein length: 1-181aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLANQLKNK K
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ARF3 ADP-ribosylation factor 3 [ Homo sapiens ]
• Official Symbol: ARF3
• Synonyms: ARF3; ADP-ribosylation factor 3; small GTP binding protein
• Gene ID: 377
• mRNA Refseq: NM_001659
• Protein Refseq: NP_001650
• MIM: 103190
• UniProt ID: P61204
• Chromosome Location: 12q13
• Function: GTP binding; GTPase activity; nucleotide binding