Recombinant Human ADIPOQ, His-tagged

Cat.No. : ADIPOQ-209H
Product Overview : Total 171AA. N-terminal His-tag and the protease cleavage site, 31 extra AA (highlighted). The AA sequence is homologous to the amino acid sequence (105-244) of the Human full-length Adiponectin.
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Species : Human
Source : E.coli
Tag : His
Protein Length : 105-244 a.a.
Description : ADIPOQ (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein which in humans is encoded by the ADIPOQ gene.
Form : Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4
Research topic : Energy metabolism and body weight regulation.
Purity : >95%
Reconstitution : Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications : Binding assay; ELISA; Ex vivo and in vivo activity analysis; In vitro; Western blotting
Quality Control Test : BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; GFC to determine purity of the protein; LAL to determine quantity of endotoxin
Sequences of amino acids : MSWWHHHHHH NWNIPTTQDT TQDLWFEGAM GGEGAYVYRS AFSVGLETYV TIPNMPIRFT KIFYNQQNHY DGSTGKFHCN IPGLYYFAYH ITVYMKDVKV SLFKKDKAML FTYDQYQENN VDQASGSVLL HLEVGDQVWL QVYGEGERNG LYADNDNDST FTGFLLYHDT N
Storage : Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Gene Name ADIPOQ adiponectin, C1Q and collagen domain containing [ Homo sapiens ]
Official Symbol ADIPOQ
Synonyms ADIPOQ; adiponectin, C1Q and collagen domain containing; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1; adiponectin; OTTHUMP00000210047; OTTHUMP00000210048; gelatin-binding protein 28; adipose specific collagen-like factor; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipose most abundant gene transcript 1 protein
Gene ID 9370
mRNA Refseq NM_004797
Protein Refseq NP_004788
MIM 605441
UniProt ID Q15848
Chromosome Location 3q27
Pathway Adipocytokine signaling pathway; Adipogenesis; Diabetes pathways; PPAR signaling pathway; Transcriptional Regulation of White Adipocyte Differentiation; Type II diabetes mellitus
Function cytokine activity; cytokine activity; hormone activity; identical protein binding; protein binding; protein homodimerization activity; receptor binding

Not For Human Consumption!

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