Recombinant Human ADIPOQ, His-tagged

• Cat.No.: ADIPOQ-209H
• Product Overview: Total 171AA. N-terminal His-tag and the protease cleavage site, 31 extra AA (highlighted). The AA sequence is homologous to the amino acid sequence (105-244) of the Human full-length Adiponectin.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 105-244 a.a.
• Description: ADIPOQ (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein which in humans is encoded by the ADIPOQ gene.
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4
• Research topic: Energy metabolism and body weight regulation.
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Binding assay; ELISA; Ex vivo and in vivo activity analysis; In vitro; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; GFC to determine purity of the protein; LAL to determine quantity of endotoxin
• Sequences of amino acids: MSWWHHHHHH NWNIPTTQDT TQDLWFEGAM GGEGAYVYRS AFSVGLETYV TIPNMPIRFT KIFYNQQNHY DGSTGKFHCN IPGLYYFAYH ITVYMKDVKV SLFKKDKAML FTYDQYQENN VDQASGSVLL HLEVGDQVWL QVYGEGERNG LYADNDNDST FTGFLLYHDT N
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Gene Information

• Gene Name: ADIPOQ adiponectin, C1Q and collagen domain containing [ Homo sapiens ]
• Official Symbol: ADIPOQ
• Synonyms: ADIPOQ; adiponectin, C1Q and collagen domain containing; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1; adiponectin; OTTHUMP00000210047; OTTHUMP00000210048; gelatin-binding protein 28; adipose specific collagen-like factor; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipose most abundant gene transcript 1 protein
• Gene ID: 9370
• mRNA Refseq: NM_004797
• Protein Refseq: NP_004788
• MIM: 605441
• UniProt ID: Q15848
• Chromosome Location: 3q27
• Pathway: Adipocytokine signaling pathway; Adipogenesis; Diabetes pathways; PPAR signaling pathway; Transcriptional Regulation of White Adipocyte Differentiation; Type II diabetes mellitus
• Function: cytokine activity; cytokine activity; hormone activity; identical protein binding; protein binding; protein homodimerization activity; receptor binding