Recombinant Human adhesion G protein-coupled receptor G1 Protein, His&Flag tagged

• Cat.No.: ADGRG1-193H
• Product Overview: Recombinant human GPR56 protein (Q9Y653-1) (Arg 26-Tyr 402) with both His, Flag tag at the C-terminus was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 26-402aa
• Description: Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination. Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12. Plays a role in the maintenance of hematopoietic stem cells and/or leukemia stem cells in bone marrow niche. Plays a critical role in cancer progression by inhibiting VEGFA production threreby inhibiting angiogenesis through a signaling pathway mediated by PRKCA. ADGRG1 NT is proposed to inhibit receptor signaling; its interactions with extracellular ligands and/or homophilic ADGRG1NT interactions may relieve the inhibition. Following ligand binding to the N-terminal fragment, the N-terminal fragment is released from the seven-transmembrane C-terminal fragment to unveil a new N-terminal stalk, which then stimulates G-protein-dependent signaling activity.
• Tag: C-His&Flag
• Molecular Mass: 45.4 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: ADGRG1 adhesion G protein-coupled receptor G1 [ Homo sapiens (human) ]
• Official Symbol: ADGRG1
• Synonyms: ADGRG1; adhesion G protein-coupled receptor G1; BFPP; BPPR; GPR56; TM7LN4; TM7XN1; CDCBM14B; CDCBM15A; adhesion G-protein coupled receptor G1; 7-transmembrane protein with no EGF-like N-terminal domains-1; G protein-coupled receptor 56; testicular tissue protein Li 77; EC 2.7.11.25
• Gene ID: 9289
• mRNA Refseq: NM_201525.4
• Protein Refseq: NP_958933.1
• MIM: 604110
• UniProt ID: Q9Y653