Recombinant Human ADAMTS5

• Cat.No.: ADAMTS5-535H
• Product Overview: Recombinant Human ADAMTS5 (Ser262­Pro622) fused 10*His tag at C terminal, was expressed in Mouse NSO cells. Recombinant Human ADAMTS5 is proneto proteolytic cleavage at C­terminus. The predominant form of the purifiedprotein lacks the His tag.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Description: ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as aggrecanase2 and ADAMTS11, is a member of the family of secreted zinc proteases with a multidomain structure. The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrinlike, TS type 1 motif, cysteinerich, spacer and a variable number of TS type 1 motifs. ADAMTS5 is an active protease effectively cleaving α2macroglobulin, aggrecan, and brevican, and is inhibited by TIMP3 with inhibition constants in the subnanomolar range. Based on the murine model studies, this protease may be a key enzyme in the degradation of cartilage leading to osteoarthritis and rheumatoid arthritis. The purified rhADAMTS5 starts at the Nterminus of the catalytic domain and ends at the Cterminus of the TSP 1 domain. The amino acid sequence of rhADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat. The aggrecanase activity can be inhibited by 5 mM 1,10phenanthroline and recombinant human TIMP3
• Predicted MolecularMass: 41 kDa
• Predicted N Terminal: Ser262
• Formulation: Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, CaCl2 and NaCl.
• Purity: >90%, by SDSPAGE under reducing conditions and visualized by silver stain.
• Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method.
• BiologicalActivity: Measured by its ability to cleave Recombinant Human Aggrecan G1IGDG2Domains
• Reconstitution: Reconstitute at 200 μg/mL in sterile, deionized water.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Stability& Storage: Use a manual defrost freezer and avoid repeated freezethaw cycles. 6 months from date of receipt, 20 to 70°C as supplied. 3 months, 20 to 70°C under sterile conditions after reconstitution.
• OfficialSymbol: ADAMTS5

Gene Information

• Gene Name: ADAMTS5 ADAM metallopeptidase with thrombospondin type 1 motif, 5 [ Homo sapiens ]
• Synonyms: ADAM metallopeptidase with thrombospondin type 1 motif, 5; ADMP-2; ADAMTS11; FLJ36738; ADAMTS5; a disintegrin and metalloproteinase with thrombospondin motifs 5; ADAM-TS5; ADAM-TS 5; ADAMTS-11; ADAM-TS 11; aggrecanase-2; a disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); EC 3.4.24.
• Gene ID: 11096
• mRNA Refseq: NM_007038
• Protein Refseq: NP_008969
• MIM: 605007
• UniProt ID: Q9UNA0
• Chromosome Location: 21q21.3
• Pathway: Endochondral Ossification
• Function: integrin binding; metalloendopeptidase activity; metallopeptidase activity; zinc ion binding