Recombinant Human ADAM17 protein, His-tagged

• Cat.No.: ADAM17-7114H
• Product Overview: Recombinant Human ADAM17 aa. (Lys226~Arg473 (Accession # P78536)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Lys226~Arg473
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.6kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: ADAM17 ADAM metallopeptidase domain 17 [ Homo sapiens (human) ]
• Official Symbol: ADAM17
• Synonyms: ADAM17; ADAM metallopeptidase domain 17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1; disintegrin and metalloproteinase domain-containing protein 17; ADAM metallopeptidase domain 18; TNF-alpha convertase; TNF-alpha converting enzyme; snake venom-like protease; tumor necrosis factor, alpha, converting enzyme
• Gene ID: 6868
• mRNA Refseq: NM_003183.5
• Protein Refseq: NP_003174.3
• UniProt ID: P78536