Recombinant Human ADA Protein, 1-363aa, N-His tagged

• Cat.No.: ADA-40H
• Product Overview: Recombinant human ADA protein (1-363aa), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-363aa
• Description: This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia.
• Form: Liquid
• Bio-activity: > 40 unit/mg, and is defined as the amount of enzyme that convert 1.0 μmol of adenosine to inosine per minute at pH 7.5 at 25 centigrade.
• Molecular Mass: 42.9 kDa (383aa) confirmed by MALDI-TOF
• AA Sequence: MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
• References: 1. Gines S., et al. (2002) Biochem. 361:203-209
  2. Daddona P.E., et al. (1984) J. Biol. Chem. 259:12101-12106

Gene Information

• Gene Name: ADA adenosine deaminase [ Homo sapiens (human) ]
• Official Symbol: ADA
• Synonyms: ADA; adenosine deaminase; ADA1; adenosine deaminase; adenosine aminohydrolase; EC 3.5.4.4
• Gene ID: 100
• mRNA Refseq: NM_000022
• Protein Refseq: NP_000013
• MIM: 608958
• UniProt ID: P00813