Recombinant Human ADA, His-tagged

• Cat.No.: ADA-1007H
• Product Overview: Recombinant human ADA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-363aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
• Molecular Weight: 42.9 kDa (383aa), confirmed by MALDI-TOF
• Purity: > 85% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Biological Activity: Specific activity: approximately >25 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme that deaminates 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25°C.
• Activity Assay: 1. Prerapre a 1.5 ml reaction mix: the final concentrations are 53.3mM potassium phosphate, 0.045mM adenosine, 0.003% (w/v) bovine serum.2. Add recombinant ADA protein with various concentrations (0.1ug, 0.2) in assay buffer.3. Mix by inversion and record A260nm for approximately 5 minutes.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ADA adenosine deaminase [ Homo sapiens ]
• Official Symbol: ADA
• Synonyms: ADA; adenosine deaminase; OTTHUMP00000031785; OTTHUMP00000240459; OTTHUMP00000240461; adenosine aminohydrolase; Adenosine aminohydrolase; EC 3.5.4.4
• Gene ID: 100
• mRNA Refseq: NM_000022
• Protein Refseq: NP_000013
• MIM: 608958
• UniProt ID: P00813
• Chromosome Location: 20q13.12
• Function: adenosine deaminase activity; hydrolase activity; metal ion binding; protein binding; purine nucleoside binding; zinc ion binding