Recombinant Human Activating Transcription Factor 1, His-tagged

• Cat.No.: ATF1-1374H
• Product Overview: Recombinanthuman ATF1protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.

Specification

• Cat. No.: ATF1-1374H
• Description: ATF1 is a cyclic-AMPdependent transcription factor. ATF1 is expressed in a wide variety of celltypes and is capable of dimerizing with CREB. MSK1 and MSK2 protein kinasesare required for the stress-induced phosphorylation of transcription factorsCREB and ATF1 in primary embryonic fibroblasts. Epidermal growth factorinduction of c-jun expression requires ATF1 and MEF2 sites in the c-junpromoter.
• Form: Liquid. In 20 mMTris-HCl buffer (pH 8.0) containing 5 mM DTT, 50% glycerol, 0.2M NaCl, 2 mM EDTA.
• Molecular Weight: 31.8 kDa(295 aa), confirmed by MALDI-TOF.
• Purity: > 90%by SDS-PAGE
• Concentration: 0.25mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMEDSHK STTSETAPQP GSAVQGAHIS HIAQQVSSLS ESEESQDSSDSIGSSQKAHG ILARRPSYRK ILKDLSSEDT RGRKGDGENS GVSAAVTSMSVPTPIYQTSS GQYIAIAPNG ALQLASPGTD GVQGLQTLTM TNSGSTQQGT TILQYAQTSD GQQILVPSNQVVVQTASGDM QTYQIRTTPS ATSLPQTVVM TSPVTLTSQT TKTDDPQLKR EIRLMKNREA ARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLY SNKSV
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: ActivatedTLR4 signalling; CREB phosphorylation; EGFR1 Signaling Pathway; ErbB1downstream signaling; HTLV-I infection; IL-3 Signaling Pathway; ImmuneSystem; Innate Immunity Signaling; MAP kinase activation in TLR cascade;MyD88 cascade initiated on plasma membrane; Myometrial Relaxation andContraction Pathways; NGF signalling via TRKA from the plasma membrane;Serotonin Receptor 4/6/7 and NR3C Signaling; Signalling by NGF; TRAF6Mediated Induction of proinflammatory cytokines; Toll Like Receptor 10(TLR10) Cascade; Toll Like Receptor 2 Cascade

Gene Information

• Gene Name: ATF1 activating transcriptionfactor 1 [ Homo sapiens ]
• Official Symbol: ATF1
• Synonyms: ATF1; activatingtranscription factor 1; TREB36; EWS-ATF1;FUS/ATF-1; cyclic AMP-dependent transcription factor ATF-1; cAMP-dependenttranscription factor ATF-1
• Gene ID: 466
• mRNA Refseq: NM_005171
• Protein Refseq: NP_005162
• MIM: 123803
• UniProt ID: P18846
• Chromosome Location: 12q13
• Function: proteincomplex binding ; protein heterodimerization activity ; sequence-specific DNAbinding ; sequence-specific DNA binding transcription factor activity ;sequence-specific distal enhancer binding RNA polymerase II transcriptionfactor activity