Recombinant Human ACTG2 protein, His & T7-tagged

• Cat.No.: ACTG2-6836H
• Product Overview: Recombinant Human ACTG2 aa. (Glu3~Phe376 (Accession # P63267)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Glu3~Phe376
• Description: Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 45.3kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.3.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: ACTG2 actin, gamma 2, smooth muscle, enteric [ Homo sapiens (human) ]
• Official Symbol: ACTG2
• Synonyms: ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG; actin-like protein; alpha-actin-3; actin, gamma-enteric smooth muscle
• Gene ID: 72
• mRNA Refseq: NM_001199893.1
• Protein Refseq: NP_001186822.1
• UniProt ID: P63267