Recombinant Human Acetyl-CoA Acyltransferase 1, His-tagged

• Cat.No.: ACAA1-784H
• Product Overview: Recombinant humanACAA1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 43.8 kDa (419aa)confirmed by MALDI-TOF.

Specification

• Cat. No.: ACAA1-784H
• Description: ACAA1 is a member of the thiolase family of enzymes and is involved in lipid metabolism. This protein is localized to the peroxisome and catalyzes the conversion of acyl-CoA and acetyl-CoA to 3-oxoacyl-CoA in the fatty acid oxidation pathway. ACAA1 shows high enzymatic activity in liver, kidney, intestine and white adipose tissue in rats. Deficiency of this enzyme leads to pseudo-Zellweger syndrome.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MLSGAPQASA ADVVVVHGRR TAICRAGRGG FKDTTPDELL SAVMTAVLKD VNLRPEQLGD ICVGNVLQPG AGAIMARIAQ FLSDIPETVP LSTVNRQCSS GLQAVASIAG GIRNGSYDIG MACGVESMSL ADRGNPGNIT SRLMEKEKAR DCLIPMGITS ENVAERFGIS REKQDTFALA SQQKAARAQS KGCFQAEIVP VTTTVHDDKG TKRSITVTQD EGIRPSTTME GLAKLKPAFK KDGSTTAGNS SQVSDGAAAI LLARRSKAEE LGLPILGVLR SYAVVGVPPD IMGIGPAYAI PVALQKAGLT VSDVDIFEIN EAFASQAAYC VEKLRLPPEK VNPLGGAVAL GHPLGCTGAR QVITLLNELK RRGKRAYGVV SMCIGTGMGA AAVFEYPGN
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ACAA1 acetyl-CoA acyltransferase 1 [ Homo sapiens ]
• Synonyms: ACAA1; acetyl-CoA acyltransferase 1; ACAA; THIO; PTHIO; 3-ketoacyl-CoA thiolase, peroxisomal; beta-ketothiolase; OTTHUMP00000209180; OTTHUMP00000209821; OTTHUMP00000209822; peroxisomal 3-oxoacyl-CoA thiolase; acetyl-Coenzyme A acyltransferase 1; peroxisomal 3-oxoacyl-Coenzyme A thiolase; EC 2.3.1.16
• Gene ID: 30
• mRNA Refseq: NM_001130410
• Protein Refseq: NP_001123882
• MIM: 604054
• UniProt ID: P09110
• Chromosome Location: 3p23-p22
• Pathway: Biosynthesis of unsaturated fatty acids; Fatty acid metabolism; Metabolic pathways; PPAR signaling pathway; KEGG pathway; Valine, leucine and isoleucine degradation
• Function: acetyl-CoA C-acyltransferase activity; acyltransferase activity; protein binding; transferase activity