Recombinant Human ACER1, GST-tagged

• Cat.No.: ACER1-9287H
• Product Overview: Recombinant Human ACER1 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: C-term-51a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: ACER1 alkaline ceramidase 1 [ Homo sapiens ]
• Official Symbol: ACER1
• Synonyms: ACER1; alkaline ceramidase 1; ASAH3, N acylsphingosine amidohydrolase (alkaline ceramidase) 3; alkCDase 1; alkaline CDase 1; acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3; N-acylsphingosine amidohydrolase (alkaline ceramidase) 3; ASAH3; ALKCDase1; MGC138327; MGC138329
• Gene ID: 125981
• mRNA Refseq: NM_133492
• Protein Refseq: NP_597999
• MIM: 613491
• UniProt ID: Q8TDN7
• Chromosome Location: 19p13.3
• Pathway: Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Sphingolipid de novo biosynthesis, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem; Sphingolipid metabolism, conserved biosystem
• Function: ceramidase activity; hydrolase activity