Recombinant Human ACE2 protein, His-Avi-tagged, Biotinylated

• Cat.No.: ACE2-2881H
• Product Overview: Biotinylated Recombinant Human ACE2 protein(Q9BYF1-1)(Gln18-Ser740) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gln18-Ser740
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized SARS-COV-2 Spike RBD, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 0.11μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• GeneID: 59272

Gene Information

• Gene Name: ACE2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Homo sapiens ]
• Official Symbol: ACE2
• Synonyms: ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; ACEH
• mRNA Refseq: NM_021804
• Protein Refseq: NP_068576
• MIM: 300335
• UniProt ID: Q9BYF1