Recombinant Human ABHD14B protein(Met1-Gln210), His-tagged

• Cat.No.: ABHD14B-7379H
• Product Overview: Recombinant Human ABHD14B (Q96IU4-1) (Met1-Gln210) was expressed in E. coli with a polyhistide tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-210 a.a.
• Form: Lyophilized from sterile PBS, 20% glycerol, 50mM Arg, 0.1% Tween20, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human ABHD14B consists of 225 amino acids and predicts a molecular mass of 24.2 KDa. It migrates as an approximately 24 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ABHD14B abhydrolase domain containing 14B [ Homo sapiens ]
• Official Symbol: ABHD14B
• Synonyms: ABHD14B; abhydrolase domain containing 14B; abhydrolase domain-containing protein 14B; CIB; MGC15429; CCG1-interacting factor B; cell cycle gene 1-interacting factor B
• Gene ID: 84836
• mRNA Refseq: NM_001146314
• Protein Refseq: NP_001139786
• UniProt ID: Q96IU4

Case Study

Case 1: Rajendran A, et al. Biochemistry. 202

The metabolic serine hydrolase family is one of the biggest enzyme groups in humans, making up about 1-2% of all proteins. These enzymes have a special serine that helps them break down various substances and include proteases, lipases, and more. There are over 250 known in humans, but a lot remain mysterious in terms of what targets they act on and what roles they have in the body. ABHD14B is part of this family and is connected to transcription processes, though its natural targets aren't fully understood. Researchers have now found that ABHD14B acts as a lysine deacetylase, meaning it can help move acetyl groups around, affecting protein functions. This study confirmed this with lab tests and cellular experiments, and they also developed a highly specific antibody to track where ABHD14B is in the body and how it might be influencing metabolism.

Fig1. Gel-based ABPP assays with WT and S111A human ABHD14B.

Fig2. WT human ABHD14B prefers hydrolysis of the acetyl group from a surrogate pNp substrate.

Case 2: Rajendran A, et al. J Biol Chem. 2022

Researchers have known sirtuins and histone deacetylases as key players in the lysine deacetylase (KDAC) enzyme family. Recently, they identified ABHD14B, another member of this gang, as a KDAC, which can transfer an acetyl-group from protein lysine to coenzyme-A, turning it into acetyl-coenzyme-A. While this is interesting, they are still piecing together how ABHD14B fits into metabolic activities. The recent research, using techniques that included cell knockdowns and analyzing gene and metabolite changes, revealed that losing ABHD14B messes with how cells handle glucose. It slows down glucose through glycolysis and the citric acid cycle, and when experimented on mice, those missing hepatic ABHD14B showed poor glucose management, especially when fasting.

Fig1. Representative Western blots confirming the knockdown of ABHD14B.

Fig2. The hepatic ATP concentrations of mice from all experimental groups.

Application

Recombinant Human ABHD14B protein is a lab-created version of an enzyme our bodies naturally make, part of a group called the alpha/beta hydrolase family. This tiny protein, with 210 amino acids and weighing about 22kDa, comes from a gene on chromosome 3. It helps break down certain compounds and might even kickstart gene activation. Recent studies suggest it acts like a lysine deacetylase in mammals, affecting how we process glucose and produce energy. Made in E. coli and tagged for easy identification, this lab-made enzyme is used in research to explore its function, metabolic role, and potential for new treatments. Understanding this protein could reveal how our bodies handle energy and signals.

Recombinant Human ABHD14B protein is making waves in research and industry due to its wide-ranging uses. In the lab, it's helping scientists dig into cell processes and protein interactions, sparking ideas for new treatments. On the industrial side, it's being looked at for designing new drugs and biotech solutions. By tapping into the abilities of ABHD14B, both fields aim to expand what's possible in health and technology.

Fig1. Putative substrates of ABHD14B. (Abinaya Rajendran, 2022)