Recombinant Human ABCA7 protein, His & GST-tagged

• Cat.No.: ABCA7-1677H
• Product Overview: Recombinant Human ABCA7 aa. (Glu53~Leu249) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This full transporter has been detected predominantly in myelo-lymphatic tissues with the highest expression in peripheral leukocytes, thymus, spleen, and bone marrow. The function of this protein is not yet known; however, the expression pattern suggests a role in lipid homeostasis in cells of the immune system.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 51.0kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Glu53~Leu249
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 5.2

Gene Information

• Gene Name: ABCA7 ATP binding cassette subfamily A member 7 [ Homo sapiens (human) ]
• Official Symbol: ABCA7
• Synonyms: ABCA7; ATP binding cassette subfamily A member 7; AD9; ABCX; ABCA-SSN; ATP-binding cassette sub-family A member 7; ATP-binding cassette, sub-family A (ABC1), member 7; autoantigen SS-N; macrophage ABC transporter
• Gene ID: 10347
• mRNA Refseq: NM_019112.3
• Protein Refseq: NP_061985.2
• UniProt ID: Q8IZY2