Recombinant Human A4GALT Protein, His-tagged

• Cat.No.: A4GALT-483H
• Product Overview: Recombinant Human A4GALT(Gly44~Leu353) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. This protein, a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor. Alternatively spliced transcript variants have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 39.1kDa
• Protein length: Gly44~Leu353
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: A4GALT alpha 1,4-galactosyltransferase [ Homo sapiens ]
• Official Symbol: A4GALT
• Synonyms: A4GALT; alpha 1,4-galactosyltransferase; alpha 1,4 galactosyltransferase (globotriaosylceramide synthase, P blood group); lactosylceramide 4-alpha-galactosyltransferase; A14GALT; CD77 synthase; Gb3 synthase; Gb3S; globotriaosylceramide synthase; P(k); GB3 synthase; alpha4Gal-T1; P1/Pk synthase; P(k) antigen synthase; P blood group (P one antigen); alpha-1,4-N-acetylglucosaminyltransferase; UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase; P1; PK; A4GALT1
• Gene ID: 53947
• mRNA Refseq: NM_017436
• Protein Refseq: NP_059132
• MIM: 607922
• UniProt ID: Q9NPC4