Recombinant Human 5'-Nucleotidase, Cytosolic II, His-tagged

• Cat.No.: NT5C2-2490H
• Product Overview: Recombinant human NT5C2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 67.1 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-561aa
• Description: NT5C2 has an essential role in the maintenance of purine/pyrimidine nucleotides. It contains a phosphotransferase active site that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5"-monophosphates. In addition, it regulates the level of inosine monophosphate (IMP) and guanosine monophosphate (GMP) pools within cells via hydrolysis.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol 0.1M NaCl,1mM DTT, 0.1mM PMSF.
• Molecular Mass: 67.1 kDa (581aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Publications:
  Ranolazine may exert its beneficial effects by increasing myocardial adenosine levels (2020)

Gene Information

• Gene Name: NT5C2 5-nucleotidase, cytosolic II [ Homo sapiens ]
• Official Symbol: NT5C2
• Synonyms: NT5C2; 5-nucleotidase, cytosolic II; 5 nucleotidase (purine), cytosolic type B , NT5B; cytosolic purine 5-nucleotidase; cN II; GMP; PNT5; purine 5 nucleotidase; IMP-specific 5-NT; cytosolic 5-nucleotidase II; 5-nucleotidase (purine), cytosolic type B; NT5B; cN-II
• Gene ID: 22978
• mRNA Refseq: NM_001134373
• Protein Refseq: NP_001127845
• MIM: 600417
• UniProt ID: P49902
• Chromosome Location: 10q24.32
• Pathway: Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Purine catabolism, organism-specific biosystem; Purine metabolism, organism-specific biosystem
• Function: 5-nucleotidase activity; hydrolase activity; metal ion binding; nucleotide binding; protein binding