Recombinant Human 3-Hydroxybutyrate Dehydrogenase, Type 1, His-tagged

• Cat.No.: BDH1-1560H
• Product Overview: Recombinant human BDH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: BDH1 is a member of the short-chain dehydrogenase/reductase gene family. This protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.2M NaCl, 2mM DTT, 0.1mM PMSF.
• Molecular Weight: 35.3 kDa (318aa), confirmed by MALDI-TOF.
• Purity: > 85% by SDS-PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MASAAEPVGS KAVLVTGCDS GFGFSLAKHL HSKGFLVFAG CLMKDKGHDG VKELDSLNSD RLRTVQLNVCSSEEVEKVVE IVRSSLKDPE KGMWGLVNNA GISTFGEVEF TSLETYKQVA EVNLWGTVRM TKSFLPLIRR AKGRVVNISS MLGRMANPARSPYCITKFGV EAFSDCLRYE MYPLGVKVSV VEPGNFIAAT SLYSPESIQA IAKKMWEELP EVVRKDYGKK YFDEKIAKME TYCSSGSTDTSPVIDAVTHA LTATTPYTRY HPMDYYWWLR MQIMTHLPGA ISDMIYIR
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Butanoate metabolism; FOXA2 and FOXA3 transcription factor networks; Fatty acid, triacylglycerol, and ketone body metabolism; Ketone body metabolism; Metabolic pathways; Metabolism of lipids and lipoproteins; Synthesis and Degradation of Ketone Bodies;Synthesis of Ketone Bodies; Utilization of Ketone Bodies

Gene Information

• Gene Name: BDH1 3-hydroxybutyrate dehydrogenase, type 1 [ Homo sapiens ]
• Official Symbol: BDH1
• Synonyms: BDH1; 3-hydroxybutyrate dehydrogenase, type 1; SDR9C1; MGC2723; MGC4347; MGC9788; D-beta-hydroxybutyrate dehydrogenase, mitochondrial; OTTHUMP00000208056; OTTHUMP00000208057; OTTHUMP00000208058; OTTHUMP00000208059; (R)-3-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase (heart, mitochondrial); short chain dehydrogenase/reductase family 9C, member 1; EC 1.1.1.30; BDH
• Gene ID: 622
• mRNA Refseq: NM_004051
• Protein Refseq: NP_004042
• MIM: 603063
• UniProt ID: Q02338
• Chromosome Location: 3q29
• Function: 3-hydroxybutyrate dehydrogenase activity; binding; oxidoreductase activity; phospholipid binding