Recombinant Human 3-alpha(17-beta)-hydroxysteroid dehydrogenase protein, His-tagged

• Cat.No.: HSD17B6-01H
• Product Overview: Recombinant human 3-alpha(17-beta)-hydroxysteroid dehydrogenase protein with C-termimal His6 fusion tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 307
• Description: The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family
• Form: Buffered aqueous solution
• Molecular Mass: 35 kDa
• AA Sequence: RLSTVRAADTIIGFEHGTAVERGTHEELLERKGVYFTLVTLQSQGNQALNEEDIKDATEDDMLARTFSRGSYQDSLRASIRQRSKSQLSYLVHEPPLAVVDHKSTYEEDRKDKDIPVQEEVEPAPVRRILK
• Purity: > 90% by SDS-PAGE
• Applications: Catalytic dehydrogenation of hydroxysteroids
• Storage: Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.145 mg/mL
• Storage Buffer: Solution in 50mM Tris-HCl, 0.3 M NaCl, pH8.0, 0.05% Sodium Lauroyl Sarcosinate

Gene Information

• Gene Name: hydroxysteroid 17-beta dehydrogenase 6 [ Homo sapiens (human) ]
• Official Symbol: HSD17B6
• Synonyms: HADH2;Endoplasmic Reticulum-Associated Amyloid Beta-Peptide-Binding Protein;3-Hydroxy-2-Methylbutyryl-CoA Dehydrogenase;AB-Binding Alcohol Dehydrogenase
• Gene ID: 8630
• mRNA Refseq: NM_003725.4
• Protein Refseq: NP_003716
• UniProt ID: Q3T001